منابع مشابه
Optimal clearing and mounting media for confocal microscopy of thick specimens
Sample preparation for confocal microscopy typically involves fixing and staining, followed by clearing and mounting. Clearing involves treatment with solvent to dissolve lipids and other structures that cause light scattering and reduce the ability to image through thick specimens. Mounting involves transfer of the specimen into a medium which matches the refractive index the objective is desi...
متن کاملPolystyrene mounting medium.
Polystyrene mounting medium obtainable Ltd, 20 Du The medium described by Kirkpatrick One pounc and Lendrum (1939) and named by them mountant DPX (Distrene plasticised in xylene) was (75°% one replaced by a better formula in 1941. This medium BPS (butyl phthalate plasticised styrene) has given excellent and economical service until recently. The current trouble was not due to the makers of the ...
متن کاملFixation, mounting and sealing with nail polish of cell specimens lead to incorrect FRET measurements using acceptor photobleaching.
Fluorescence resonance energy transfer (FRET) is a technique used for the study of functional interactions between molecules. The intimate vicinity between two fluorescent molecules (FRET-pair; donor and acceptor) allows for an energy transfer, which can be directly calculated as the so called FRET efficiency. This technique is used in fixed as well as living cells. Here we show first, measured...
متن کاملPathologic observations of the duodenum in 615 consecutive duodenal specimens: I. benign lesions.
The author investigated histopathology of 615 consecutive duodenal specimens in our pathology laboratory. Computer search of the duodenal lesions was performed. Review of histological slides was done, when appropriate. The duodenal specimens were composed of 567 benign lesions and 48 malignant lesions. The 567 benign lesions were composed of chronic non-specific duodenitis in 334 cases (60.0%),...
متن کاملValue-based analysis of routine pathologic septal and inferior turbinate specimens.
OBJECTIVE To determine the frequency and clinical relevance of unanticipated histopathologic results in routine sinonasal surgery and evaluate the necessity for histologic processing of nasal septal cartilage, bone, and inferior turbinate specimens. STUDY DESIGN Case series with chart review. SETTING Tertiary care academic medical center. SUBJECTS AND METHODS A retrospective review of sur...
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ژورنال
عنوان ژورنال: Journal of the American Medical Association
سال: 1912
ISSN: 0002-9955
DOI: 10.1001/jama.1912.04260010217027